7N8L

PptT PAP(CoA) 9016 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	120
Detector technology	PIXEL
Collection date	2017-12-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	134.746, 63.474, 79.868
Unit cell angles	90.00, 123.51, 90.00

Refinement procedure

Resolution	37.780 - 2.260
R-factor	0.2103
Rwork	0.209
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ct5
RMSD bond length	0.003
RMSD bond angle	0.559
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_3126)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.780	2.340
High resolution limit [Å]	2.260	2.260
Rmerge		0.666
Number of reflections	26336	2608
<I/σ(I)>	8.5
Completeness [%]	99.2
Redundancy	4.9
CC(1/2)	0.923	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	291.15	0.1 M Bis-Tris Propane pH 6.7, 1.8M Magnesium Sulfate