7N8G
Crystal structure of R20A-R22A human Galectin-7 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.240, 64.960, 72.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.280 - 1.950 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.039 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.7.12) |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.290 | 48.290 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.070 | 0.057 | 0.229 |
| Rmeas | 0.074 | 0.060 | 0.242 |
| Rpim | 0.024 | 0.020 | 0.076 |
| Number of reflections | 18712 | 2018 | 1791 |
| <I/σ(I)> | 22.6 | 34.2 | 10 |
| Completeness [%] | 99.5 | 99.9 | 98.8 |
| Redundancy | 9.7 | 9.1 | 9.9 |
| CC(1/2) | 0.998 | 0.998 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000 ,17.5% Glycerol |
