1BKZ

CRYSTAL STRUCTURE OF HUMAN GALECTIN-7

Summary for 1BKZ

• Entry DOI: 10.2210/pdb1bkz/pdb
• Descriptor: GALECTIN-7 (2 entities in total)
• Functional Keywords: galaptin, lectin, galectin, carbohydrate binding
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 29931.70

Authors

Leonidas, D.D.,Acharya, K.R. (deposition date: 1998-07-13, release date: 1998-11-04, Last modification date: 2024-02-07)

Primary citation

Leonidas, D.D.,Vatzaki, E.H.,Vorum, H.,Celis, J.E.,Madsen, P.,Acharya, K.R.
Structural basis for the recognition of carbohydrates by human galectin-7.
Biochemistry, 37:13930-13940, 1998

PubMed Abstract: Knowledge about carbohydrate recognition domains of galectins, formerly known as S-type animal lectins, is important in understanding their role(s) in cell-cell interactions. Here we report the crystal structure of human galectin-7 (hGal-7), in free form and in the presence of galactose, galactosamine, lactose, and N-acetyl-lactosamine at high resolution. This is the first structure of a galectin determined in both free and carbohydrate-bound forms. The structure shows a fold similar to that of the prototype galectins -1 and -2, but has greater similarity to a related galectin molecule, Gal-10. Even though the carbohydrate-binding residues are conserved, there are significant changes in this pocket due to shortening of a loop structure. The monomeric hGal-7 molecule exists as a dimer in the crystals, but adopts a packing arrangement considerably different from that of Gal-1 and Gal-2, which has implications for carbohydrate recognition.

PubMed: 9760227
DOI: 10.1021/bi981056x

Experimental method

X-RAY DIFFRACTION (1.9 Å)