7N37

Crystal structure of 3-site deamidated variant of human gamma(S)-crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	29.449, 62.552, 79.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.620 - 1.300
R-factor	0.1469
Rwork	0.145
R-free	0.17930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fd8
RMSD bond length	0.014
RMSD bond angle	1.305
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.620	1.350
High resolution limit [Å]	1.300	1.300
Number of reflections	36161	3477
<I/σ(I)>	27.64
Completeness [%]	97.6
Redundancy	13.1
CC(1/2)	1.000	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M MgCl2, 0.1 M BIS-TRIS pH 5.5, 25 % w/v Polyethylene glycol 3350