7N0A
Structure of Human Leukaemia Inhibitory Factor with Fab MSC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332013463416092 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.572, 109.380, 115.691 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.740 - 3.100 |
R-factor | 0.2345 |
Rwork | 0.232 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pvh |
RMSD bond length | 0.002 |
RMSD bond angle | 0.534 |
Data reduction software | XPREP |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.740 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 14528 | 1427 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 98.4 | |
Redundancy | 12 | |
CC(1/2) | 0.975 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277.15 | 19% (v/v) isopropanol, 19% (w/v) PEG 4000, 5% (v/v) glycerol, 0.095 M sodium citrate, pH 5.6 |