7MSE
High-resolution crystal structure of hMIF2 with tartrate at the active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-30 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.51418 |
Spacegroup name | P 3 |
Unit cell lengths | 83.396, 83.396, 40.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.700 - 1.270 |
R-factor | 0.1388 |
Rwork | 0.137 |
R-free | 0.17290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kan |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.290 |
High resolution limit [Å] | 1.270 | 3.450 | 1.270 |
Rmerge | 0.077 | 0.033 | 0.605 |
Rmeas | 0.080 | 0.034 | 0.701 |
Rpim | 0.023 | 0.008 | 0.341 |
Total number of observations | 933698 | ||
Number of reflections | 82635 | 4158 | 3642 |
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.3 | 100 | 86.8 |
Redundancy | 11.3 | 19.1 | 3.4 |
CC(1/2) | 1.000 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 28.6% w/v PEG 3350, 0.31M sodium tartrate at pH 5.6 |