7MRZ
Structure of GDF11 bound to fused ActRIIB-ECD and Alk4-ECD with Anti-ActRIIB Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033202 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 68.261, 68.261, 990.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.060 - 3.000 |
| R-factor | 0.2902 |
| Rwork | 0.288 |
| R-free | 0.32710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mac |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.631 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.510 | 49.510 | 3.180 |
| High resolution limit [Å] | 3.000 | 9.000 | 3.000 |
| Rmerge | 0.181 | 0.146 | 0.315 |
| Rmeas | 0.189 | 0.150 | 0.356 |
| Rpim | 0.052 | 0.035 | 0.160 |
| Total number of observations | 237557 | 21760 | 19190 |
| Number of reflections | 29791 | 1422 | 4493 |
| <I/σ(I)> | 8.1 | 14.1 | 2.8 |
| Completeness [%] | 99.0 | 99.1 | 96.8 |
| Redundancy | 8 | 15.3 | 4.3 |
| CC(1/2) | 0.992 | 0.992 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 0.9M ammonium sulfate, 0.9 M KCl |
