7MRV
F100A mutant structure of MIF2 (D-DT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-08 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.514 |
Spacegroup name | H 3 |
Unit cell lengths | 83.161, 83.161, 37.781 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.580 - 1.570 |
R-factor | 0.1693 |
Rwork | 0.167 |
R-free | 0.21110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kan |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.600 |
High resolution limit [Å] | 1.570 | 4.260 | 1.570 |
Rmerge | 0.030 | 0.032 | 0.129 |
Rmeas | 0.033 | 0.034 | 0.152 |
Rpim | 0.012 | 0.011 | 0.079 |
Total number of observations | 70639 | ||
Number of reflections | 13566 | 683 | 688 |
<I/σ(I)> | 31.4 | ||
Completeness [%] | 99.9 | 100 | 98.4 |
Redundancy | 5.2 | 8.9 | 3.3 |
CC(1/2) | 0.999 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M Ammonium sulfate, 25% PEG3350, 0.1M Tris |