7MNW

Crystal Structure of Nup358/RanBP2 Ran-binding domain 1 in complex with Ran-GPPNHP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	137.370, 137.834, 172.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.960 - 2.400
R-factor	0.2016
Rwork	0.200
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rrp
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.960	28.960	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmeas	0.306	0.122	2.847
Rpim	0.082	0.034	0.750
Total number of observations	880269	89717	87689
Number of reflections	64146	6687	6219
<I/σ(I)>	6.7	13.9	1.3
Completeness [%]	99.8	100	98.2
Redundancy	13.7	13.4	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% w/v PEG3350, 0.2 M potassium sodium tartrate tetrahydrate