7MKX
Crystal Structure Analysis of human CDK2 and CCNA2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 161.050, 161.520, 65.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.760 - 3.080 |
R-factor | 0.2476 |
Rwork | 0.246 |
R-free | 0.28880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h1r |
RMSD bond length | 0.002 |
RMSD bond angle | 0.602 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.760 | 80.790 | 3.130 |
High resolution limit [Å] | 3.080 | 8.360 | 3.080 |
Rmeas | 0.195 | 0.038 | 2.536 |
Rpim | 0.075 | 0.015 | 0.958 |
Total number of observations | 218526 | 10611 | 11151 |
Number of reflections | 32528 | 1788 | 1615 |
<I/σ(I)> | 9.3 | 34.2 | 1.2 |
Completeness [%] | 99.8 | 100 | 99.6 |
Redundancy | 6.7 | 5.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5M Ammonium Citrate, pH 6.0 |