7MKX

Crystal Structure Analysis of human CDK2 and CCNA2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	161.050, 161.520, 65.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.760 - 3.080
R-factor	0.2476
Rwork	0.246
R-free	0.28880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h1r
RMSD bond length	0.002
RMSD bond angle	0.602
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.760	80.790	3.130
High resolution limit [Å]	3.080	8.360	3.080
Rmeas	0.195	0.038	2.536
Rpim	0.075	0.015	0.958
Total number of observations	218526	10611	11151
Number of reflections	32528	1788	1615
<I/σ(I)>	9.3	34.2	1.2
Completeness [%]	99.8	100	99.6
Redundancy	6.7	5.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.5M Ammonium Citrate, pH 6.0