7MBH
Structure of Human Enolase 2 in complex with phosphoserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.050, 108.380, 117.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.390 - 2.100 |
| R-factor | 0.1829 |
| Rwork | 0.180 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zcw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.876 |
| Data reduction software | MOSFLM (7.2.2) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.1-4122-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.390 | 42.390 | 2.160 |
| High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
| Rmerge | 0.392 | 0.091 | 4.433 |
| Rmeas | 0.410 | 0.095 | 4.654 |
| Rpim | 0.117 | 0.028 | 1.375 |
| Total number of observations | 564387 | 8720 | 37878 |
| Number of reflections | 50068 | 770 | 3840 |
| <I/σ(I)> | 7.2 | 19.3 | 1.5 |
| Completeness [%] | 97.3 | 98.8 | 92.7 |
| Redundancy | 11.3 | 11.3 | 9.9 |
| CC(1/2) | 0.989 | 0.990 | 0.325 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Bis Tris, 200 mM ammonium acetate and 21% (w/v) PEG 3350 at pH 6.5 |
