7M8H
Structure of Memo1 C244S metal binding site mutant at 1.75A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-12 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.968 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 140.000, 89.520, 97.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.630 - 1.750 |
R-factor | 0.1556 |
Rwork | 0.154 |
R-free | 0.18720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kq8 |
Data reduction software | AutoProcess |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.630 | 42.630 | 1.860 |
High resolution limit [Å] | 1.750 | 5.000 | 1.750 |
Rmerge | 0.088 | 0.026 | 1.052 |
Rmeas | 0.091 | 0.027 | 1.091 |
Number of reflections | 238611 | 5654 | 20426 |
<I/σ(I)> | 27.83 | 75.54 | 3.2 |
Completeness [%] | 99.9 | 99.7 | 99.8 |
Redundancy | 14.819 | 14.169 | 14.272 |
CC(1/2) | 1.000 | 1.000 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 0.1 M MES pH 5.5-7.0 22 % PEG3350 under anaerobic conditions |