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7M8H

Structure of Memo1 C244S metal binding site mutant at 1.75A

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCLSI BEAMLINE 08B1-1
Synchrotron siteCLSI
Beamline08B1-1
Temperature [K]100
Detector technologyCCD
Collection date2020-03-12
DetectorRAYONIX MX-300
Wavelength(s)0.968
Spacegroup nameP 21 21 2
Unit cell lengths140.000, 89.520, 97.540
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution42.630 - 1.750
R-factor0.1556
Rwork0.154
R-free0.18720
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)7kq8
Data reduction softwareAutoProcess
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwarePHENIX (1.19.1_4122)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]42.63042.6301.860
High resolution limit [Å]1.7505.0001.750
Rmerge0.0880.0261.052
Rmeas0.0910.0271.091
Number of reflections238611565420426
<I/σ(I)>27.8375.543.2
Completeness [%]99.999.799.8
Redundancy14.81914.16914.272
CC(1/2)1.0001.0000.893
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.52920.1 M MES pH 5.5-7.0 22 % PEG3350 under anaerobic conditions

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