7M60

Structure of Mouse Importin alpha MLH1-S467A NLS Peptide Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.425
Spacegroup name	P 21 21 21
Unit cell lengths	78.440, 90.049, 99.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.043 - 2.300
R-factor	0.1753
Rwork	0.174
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u5p
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.043	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.088	0.976
Rmeas	0.092	1.022
Rpim	0.027	0.297
Number of reflections	345941	3024
<I/σ(I)>	24.37	2.72
Completeness [%]	98.1	96.31
Redundancy	11	11.3
CC(1/2)	0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1M sodium citrate pH6, 0.65 M sodium citrate, 10 mM DTT