7M2O
Crystal structure of Human Glycolate Oxidase with Inhibitor Compound 15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-31 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 96.904, 96.904, 79.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.770 - 2.070 |
| R-factor | 0.12793 |
| Rwork | 0.126 |
| R-free | 0.16607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rdw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.754 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.095 | |
| Number of reflections | 22486 | 1133 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6 | |
| CC(1/2) | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris-HCL pH 8.5, 25 % (w/v) PEG 1000 |
