7M2G

INTERLEUKIN-2 (human) mutant P65K, C125S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999965
Spacegroup name	P 43 21 2
Unit cell lengths	73.501, 73.501, 76.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.160 - 1.790
R-factor	0.1958
Rwork	0.192
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qb1
RMSD bond length	0.015
RMSD bond angle	1.708
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.160	1.819
High resolution limit [Å]	1.788	1.788
Rmerge	0.048	1.866
Rmeas	0.050	1.439
Rpim	0.013	0.358
Number of reflections	20517	2930
<I/σ(I)>	27.8	1.6
Completeness [%]	100.0	100
Redundancy	16.1	15.7
CC(1/2)	0.998	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.75	293	1.75M (NH4)2SO4, 0.10 M HEPES/NaOH pH=7.75 at a protein concentration of 37 mg/ml.