7M0L
HPK1 IN COMPLEX WITH COMPOUND 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.850, 76.723, 88.413 |
| Unit cell angles | 89.97, 97.84, 90.03 |
Refinement procedure
| Resolution | 87.590 - 2.430 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.29280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PREVIOUSLY SOLVED STRUCTURE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.572 |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.590 | 2.680 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmeas | 0.057 | 0.563 |
| Number of reflections | 42306 | 10850 |
| <I/σ(I)> | 12.89 | 2.99 |
| Completeness [%] | 96.1 | 96.2 |
| Redundancy | 2.2 | 2.1 |
| CC(1/2) | 0.999 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20 mM Tris pH 8.0 , 150 mM NaCl , 5% Glycerol , 2 mM DTT |
