7M06
Dihydrodipicolinate synthase (DHDPS) from C.jejuni, Y110F mutant with R,R-bislysine bound at the allosteric site at 2.7 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.030, 84.290, 200.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.150 - 2.700 |
R-factor | 0.1961 |
Rwork | 0.194 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ly8 |
Data reduction software | HKL-3000 |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.150 | 2.797 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.035 | 0.283 |
Rmeas | 0.050 | 0.400 |
Number of reflections | 34342 | 3362 |
<I/σ(I)> | 17.29 | 2.82 |
Completeness [%] | 99.7 | 99.82 |
Redundancy | 14 | |
CC(1/2) | 0.998 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 288.15 | 0.2 M Magnesium acetate, 10% PEG 8000 |