7LVB
CHOLERA TOXIN B SUBUNIT WITH ATTACHED SIV EPITOPE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.290, 112.428, 137.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 2.250 |
| R-factor | 0.1577 |
| Rwork | 0.157 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ct1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.260 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.118 | 0.880 |
| Number of reflections | 62497 | 7191 |
| <I/σ(I)> | 13.3 | 2.4 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 10.2 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 18-24% PEG3350, 0.1M TRIS-HCL, PH 8.0, 10MM GALACTOSE |
