7LUS

IgG2 Fc Charge Pair Mutation version 1 (CPMv1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-06
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	112.584, 112.584, 99.417
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.985 - 2.450
R-factor	0.1842
Rwork	0.181
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4haf
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.450	5.280	2.450
Rmerge	0.088	0.042	0.961
Rmeas	0.090	0.045
Rpim	0.033	0.016	0.402
Number of reflections	26116	2712	2369
<I/σ(I)>	8.4
Completeness [%]	98.8	99.9	90
Redundancy	7.9	8.2	5.8
CC(1/2)		0.999	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	12.5% w/v PEG550MME, 12.5% w/v PEG20K, 0.1 M Tris-HCl (pH 8.5), 20% 2,3-butanediol, 3% ethanol