7LUR

Stable Effector Functionless 2 (SEFL2) IgG1 Fc Scaffold Bound to a Minimized Version of the B-domain (Mini-Z) from Protein A Called Z34C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-17
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	63.438, 69.335, 135.421
Unit cell angles	90.00, 98.64, 90.00

Refinement procedure

Resolution	40.360 - 1.950
R-factor	0.218
Rwork	0.216
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l6x
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.510	46.510	2.000
High resolution limit [Å]	1.950	8.940	1.950
Rmerge	0.246	0.080	1.897
Total number of observations	283884	3065	20377
Number of reflections	42098	467	2932
<I/σ(I)>	6.9	22.7	1.3
Completeness [%]	99.3	98.8	98.5
Redundancy	6.7	6.6	6.9
CC(1/2)	0.991	0.991	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.2 M ammonium tartrate, 20% (w/v) PEG 3350, 10% NDSB-221