7LTT
SAMHD1(113-626) H206R D207N R366C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.926, 140.105, 97.193 |
Unit cell angles | 90.00, 114.18, 90.00 |
Refinement procedure
Resolution | 39.380 - 1.900 |
R-factor | 0.1792 |
Rwork | 0.177 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bzb |
RMSD bond length | 0.012 |
RMSD bond angle | 1.698 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.071 | 1.311 |
Number of reflections | 153730 | 7144 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 98.9 | 98.8 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.996 | 0.297 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.2 | 293 | PEG 1500, dGTP, SPG buffer, sodium chloride, magnesium chloride, TCEP |