7LSF

Crystal structure of the human neutralizing antibody Fab fragment T025 bound to TBEV EDIII (Western Subtype)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	55.542, 66.730, 91.210
Unit cell angles	90.00, 94.64, 90.00

Refinement procedure

Resolution	90.910 - 2.240
R-factor	0.1912
Rwork	0.189
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ghw 4ogx 6j5f
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.910	2.320
High resolution limit [Å]	2.240	2.240
Number of reflections	31036	2897
<I/σ(I)>	8.8
Completeness [%]	97.2	98
Redundancy	6	6
CC(1/2)	0.994	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.1M sodium citrate tribasic dihydrate pH 5.0, 10% PEG 6000