7LSF
Crystal structure of the human neutralizing antibody Fab fragment T025 bound to TBEV EDIII (Western Subtype)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.542, 66.730, 91.210 |
Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
Resolution | 90.910 - 2.240 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ghw 4ogx 6j5f |
RMSD bond length | 0.007 |
RMSD bond angle | 0.952 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.910 | 2.320 |
High resolution limit [Å] | 2.240 | 2.240 |
Number of reflections | 31036 | 2897 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 97.2 | 98 |
Redundancy | 6 | 6 |
CC(1/2) | 0.994 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 0.1M sodium citrate tribasic dihydrate pH 5.0, 10% PEG 6000 |