7LQL

Crystal structure of the R375D mutant of LeuT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	86.629, 87.059, 80.589
Unit cell angles	90.00, 95.32, 90.00

Refinement procedure

Resolution	43.530 - 2.600
R-factor	0.1951
Rwork	0.193
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f3e
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.220	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	18238	2210
<I/σ(I)>	7.6
Completeness [%]	99.0
Redundancy	7.1
CC(1/2)	0.990	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	100 mM Hepes/NaOH pH 7.0, 200 mM NaCl, 20-22% PEG 550 MME