7LMO
Structure of full-length human lambda-6A light chain JTO in complex with stabilizer 34 [3-(2-(7-(diethylamino)-4-methyl-2-oxo-2H-chromen-3-yl)ethyl)-7-(1H-imidazole-5-carbonyl)-1,3,7-triazaspiro[4.4]nonane-2,4-dione]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.884, 82.846, 95.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.740 - 1.990 |
R-factor | 0.1784 |
Rwork | 0.176 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mg5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.544 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.740 | 2.100 |
High resolution limit [Å] | 1.990 | 1.990 |
Rpim | 0.035 | 0.298 |
Number of reflections | 35344 | 5011 |
<I/σ(I)> | 13.3 | 2.5 |
Completeness [%] | 99.8 | 98.7 |
Redundancy | 12.3 | 12.4 |
CC(1/2) | 1.000 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 20% PEG 3350 and 0.25 M NH4H2PO4 at 23 degrees C |