7LMM
Crystal structure of bovine DNMT1 BAH1 domain in complex with H4K20me2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 70.696, 80.281, 130.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.906 - 2.798 |
R-factor | 0.2125 |
Rwork | 0.207 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wxx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.962 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.910 | 2.900 |
High resolution limit [Å] | 2.790 | 2.800 |
Number of reflections | 18810 | 1823 |
<I/σ(I)> | 20.8 | |
Completeness [%] | 99.5 | |
Redundancy | 12.9 | |
CC(1/2) | 0.998 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M MES (pH 6.5) ,13% PEG1500, 20 mM DTT and 200 mM L-proline |