7LM8

Crystal structure of SARS-CoV-2 spike protein receptor-binding domain in complex with two cross-neutralizing antibodies CV38-142 and COVA1-16 Fabs isolated from COVID-19 patients

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	59.664, 148.234, 162.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.708 - 1.936
R-factor	0.1703
Rwork	0.169
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jmw
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.936	5.260	1.940
Rmerge	0.086	0.038	0.873
Rmeas	0.090	0.040	0.933
Rpim	0.028	0.013	0.322
Number of reflections	107834	5782	5287
<I/σ(I)>	6
Completeness [%]	100.0	100	100
Redundancy	10.2	9.9	8.3
CC(1/2)		0.998	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	10% (w/v) polyethylene glycol 6000, 0.1 M citric acid pH 4.0