7LIU
DDX3X bound to ATP analog and remodeled RNA:DNA hybrid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2016-08-09 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 114.476, 114.476, 281.793 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.540 - 3.001 |
R-factor | 0.2112 |
Rwork | 0.210 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pxa |
RMSD bond length | 0.008 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmeas | 0.121 | 0.964 |
Rpim | 0.044 | 0.379 |
Number of reflections | 43593 | 4265 |
<I/σ(I)> | 20.7 | 1.6 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 7 | 6.2 |
CC(1/2) | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPEs pH 7.5, 16% PEG 3350, 0.1 M Li3Citrate, 0.1 M Na3Citrate, 2% benzamidine-HCl, 5 mM ADP-BeF3 |