7LEQ

Structure of importin a2 bound to p50 NLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	78.187, 90.730, 97.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.890 - 2.240
R-factor	0.191
Rwork	0.189
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y2a
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.330
High resolution limit [Å]	2.240	2.240
Number of reflections	27626	2882
<I/σ(I)>	14.4	1.5
Completeness [%]	81.9	87.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.6M sodium citrate, 100 Hepes pH 6.0