7LD9
Structure of human GGT1 in complex with ABBA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.319, 125.570, 104.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.690 - 1.420 |
| R-factor | 0.108 |
| Rwork | 0.107 |
| R-free | 0.13400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4z9o |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.654 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.8.0073) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.000 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.052 | 0.714 |
| Number of reflections | 128369 | 5352 |
| <I/σ(I)> | 25.57 | 2.44 |
| Completeness [%] | 98.9 | 83.1 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 20-25%PEG3350, 0.1M ammonium cloride, 0.1M sodium cacodilate pH 6.0 |
