7L9I

Crystal structure of human ARH3-D314A bound to magnesium and ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	44.808, 71.428, 115.803
Unit cell angles	94.03, 94.63, 107.86

Refinement procedure

Resolution	67.640 - 1.800
R-factor	0.1908
Rwork	0.189
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d36
RMSD bond length	0.006
RMSD bond angle	0.848
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.640	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.120	0.550
Number of reflections	112761	11030
<I/σ(I)>	4.6
Completeness [%]	89.8
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M sodium acetate buffer pH 4.5, 0.1 M MgCl2, and 24 % polyethylene glycol (PEG) 4000