7L9F

Crystal structure of human ARH3 bound to calcium and ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	44.930, 71.473, 115.595
Unit cell angles	93.97, 94.33, 107.41

Refinement procedure

Resolution	67.880 - 1.750
R-factor	0.1512
Rwork	0.149
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d36
RMSD bond length	0.009
RMSD bond angle	1.097
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.880	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.028	0.117
Number of reflections	125732	12401
<I/σ(I)>	20
Completeness [%]	91.5
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M sodium acetate buffer pH 4.5, 0.1 M CaCl2, and 24 % polyethylene glycol (PEG) 4000