7L5B

Crystallographic structure of neutralizing antibody 2-15 in complex with SARS-CoV-2 spike receptor-binding Domain (RBD).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	I 1 2 1
Unit cell lengths	134.013, 70.439, 140.039
Unit cell angles	90.00, 96.93, 90.00

Refinement procedure

Resolution	102.490 - 3.180
R-factor	0.1919
Rwork	0.187
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bz5
RMSD bond length	0.010
RMSD bond angle	1.410
Data reduction software	XDS
Data scaling software	Aimless (V.7.0)
Phasing software	PHENIX ((1.18.2_3874: ???))
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.490	3.880
High resolution limit [Å]	3.180	3.180
Rmerge	0.111	0.110
Number of reflections	27227	4388
<I/σ(I)>	1.7
Completeness [%]	99.8
Redundancy	4.5
CC(1/2)	0.951	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M Hepes, 70 % MPD