7L2C
Crystallographic structure of neutralizing antibody 2-51 in complex with SARS-CoV-2 spike N-terminal domain (NTD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.791, 115.785, 137.606 |
| Unit cell angles | 90.00, 99.97, 90.00 |
Refinement procedure
| Resolution | 88.030 - 3.650 |
| R-factor | 0.2191 |
| Rwork | 0.216 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7c2l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.481 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.030 | 3.650 |
| High resolution limit [Å] | 3.440 | 3.440 |
| Number of reflections | 25964 | 5916 |
| <I/σ(I)> | 2.57 | |
| Completeness [%] | 93.3 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.883 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.16 M Calcium Acetate, 0.08 M Sodium Cacodylate, 14.4% PEG 8000, 20% Glycerol |
