7KXT
Crystal structure of human EED
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.470, 49.940, 105.320 |
| Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
| Resolution | 40.390 - 2.150 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jzn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.932 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.392 | 40.390 | 2.210 |
| High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
| Rmerge | 0.104 | 0.035 | 0.813 |
| Rmeas | 0.123 | 0.043 | 0.982 |
| Rpim | 0.066 | 0.024 | 0.541 |
| Total number of observations | 1568 | 9660 | |
| Number of reflections | 44346 | 533 | 3122 |
| <I/σ(I)> | 8.2 | 24.1 | 1.4 |
| Completeness [%] | 98.7 | 96.7 | 94.6 |
| Redundancy | 3.4 | 2.9 | 3.1 |
| CC(1/2) | 0.995 | 0.996 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 3.5M Sodium Formate, 0.1M TrisCl pH8.5 |
