7KXT

Crystal structure of human EED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	81.470, 49.940, 105.320
Unit cell angles	90.00, 105.11, 90.00

Refinement procedure

Resolution	40.390 - 2.150
R-factor	0.1798
Rwork	0.178
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jzn
RMSD bond length	0.008
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.392	40.390	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.104	0.035	0.813
Rmeas	0.123	0.043	0.982
Rpim	0.066	0.024	0.541
Total number of observations		1568	9660
Number of reflections	44346	533	3122
<I/σ(I)>	8.2	24.1	1.4
Completeness [%]	98.7	96.7	94.6
Redundancy	3.4	2.9	3.1
CC(1/2)	0.995	0.996	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	3.5M Sodium Formate, 0.1M TrisCl pH8.5