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7KX1

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, Y110F mutant with pyruvate bound in the active in C2221 space group

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCLSI BEAMLINE 08ID-1
Synchrotron siteCLSI
Beamline08ID-1
Temperature [K]100
Detector technologyPIXEL
Collection date2019-10-29
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)0.9795
Spacegroup nameC 2 2 21
Unit cell lengths83.810, 230.100, 199.960
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution45.850 - 2.040
R-factor0.2155
Rwork0.213
R-free0.25380
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4ly8
Data reduction softwareHKL-3000
Data scaling softwareXSCALE
Phasing softwareMOLREP
Refinement softwarePHENIX (1.17.1_3660)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]46.0301.968
High resolution limit [Å]1.9001.900
Rmerge1.077
Number of reflections15629415421
<I/σ(I)>8.43
Completeness [%]99.999.59
Redundancy15
CC(1/2)0.990
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1MICROBATCH7.4288.150.3 M Magnesium acetate, 10 % PEG 8000, 0.1 M Sodium acetate (pH 7.4)

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