7KQN

Ternary complex of TERT (telomerase reverse transcriptase) with RNA template, DNA primer, an incoming dGTP and a downstream hybrid duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-27
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	71.860, 164.040, 95.360
Unit cell angles	90.00, 111.91, 90.00

Refinement procedure

Resolution	29.650 - 2.020
R-factor	0.1677
Rwork	0.168
R-free	0.18780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kyl
RMSD bond length	0.010
RMSD bond angle	1.228
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.130
High resolution limit [Å]	2.020	2.020
Number of reflections	131028	9762
<I/σ(I)>	10.55	1.07
Completeness [%]	97.9
Redundancy	3.7
CC(1/2)	0.998	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.15 M Potassium Chloride, 0.05 M MES (pH 6.0), 2% PEG 8000