7KQK
anti-pTau C21-ABS Fab in complex with pTau peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-07-15 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.370, 76.580, 119.470 |
Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
Resolution | 64.470 - 2.600 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4glr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.600 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.079 | 0.410 |
Number of reflections | 29183 | 4234 |
<I/σ(I)> | 9.5 | |
Completeness [%] | 99.7 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 20 % PEG 3350 (w/v) and 150 mM DL-malic acid. |