Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

7KK1

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, N84A mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCLSI BEAMLINE 08ID-1
Synchrotron siteCLSI
Beamline08ID-1
Temperature [K]100
Detector technologyCCD
Collection date2019-02-09
DetectorRAYONIX MX300-HS
Wavelength(s)0.9795
Spacegroup nameC 2 2 21
Unit cell lengths85.690, 231.980, 200.450
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.810 - 1.770
R-factor0.1697
Rwork0.168
R-free0.19860
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4ly8
Data reduction softwareHKL-3000
Data scaling softwareXSCALE
Phasing softwareAMPLE
Refinement softwarePHENIX (1.17.1_3660)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.8101.761
High resolution limit [Å]1.6501.650
Rmerge0.752
Number of reflections23816320845
<I/σ(I)>9.56
Completeness [%]100.099.7
Redundancy15
CC(1/2)0.990
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1MICROBATCH7.4288.150.1 M Magnesium acetate, 12 % PEG 8000, 0.1 M Sodium acetate (pH 7.4), 60 mM L-lysine

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon