7KJC

Crystal structure of the EphA2 S901E mutant intracellular KD-SAM domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	2014-12-19
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	I 1 2 1
Unit cell lengths	121.639, 54.587, 135.538
Unit cell angles	90.00, 95.22, 90.00

Refinement procedure

Resolution	29.250 - 2.300
R-factor	0.1903
Rwork	0.188
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pdo 3kka
RMSD bond length	0.002
RMSD bond angle	0.520
Data reduction software	XDS (VERSION January 10, 2014)
Data scaling software	Aimless (version 0.5.32)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.250	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.656
Rmeas	0.125	0.905
Rpim	0.086	0.621
Number of reflections	39708	3887
<I/σ(I)>	12	1.8
Completeness [%]	99.8
Redundancy	3.7
CC(1/2)	0.995	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	287	0.2 M MgCl2, 0.1 M Bis-Tris pH5.5, 25% PEG 3350