7KHT

The acyl chains of phosphoinositide PIP3 alter the structure and function of nuclear receptor Steroidogenic Factor-1 (SF-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-20
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 41 21 2
Unit cell lengths	65.349, 65.349, 139.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.537 - 2.504
R-factor	0.2202
Rwork	0.219
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qjr
RMSD bond length	0.010
RMSD bond angle	1.297
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.540	2.594
High resolution limit [Å]	2.500	2.504
Number of reflections	21999	1053
<I/σ(I)>	21.8	2.6
Completeness [%]	98.4	98.78
Redundancy	2	2
CC(1/2)	1.000	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350 and 0.2 M magnesium formate