7KFY
Structural basis for a germline-biased antibody response to SARS-CoV-2 (RBD:C1A-F10 Fab)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-08-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 85.740, 146.801, 144.593 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.040 - 2.157 |
R-factor | 0.2078 |
Rwork | 0.205 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kfv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.000 |
Data reduction software | XDS (20200131) |
Data scaling software | XDS (20200131) |
Phasing software | PARROT (2.8.3) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 2.290 |
High resolution limit [Å] | 2.157 | 2.157 |
Rmerge | 0.118 | |
Rmeas | 0.157 | |
Number of reflections | 48373 | 27507 |
<I/σ(I)> | 6.17 | 0.71 |
Completeness [%] | 94.0 | 89.4 |
Redundancy | 2.06 | 1.96 |
CC(1/2) | 0.991 | 0.370 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | protein sample: 13 mg/mL in 150mM NaCl, 25 mM Tris-HCl, pH 7.5 mother liquor composition (equal volume): 0.10% (w/v) n-Octyl-B-glucoside, 0.1 M Sodium citrate tribasic dihydrate pH 4.5, 22% (w/v) PEG 3350 |