7KEZ
Crystal structure of bH1 Fab variant (CDR H3 loop design 16_0325) in complex with VEGF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.799, 193.839, 76.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.920 - 2.310 |
| R-factor | 0.1806 |
| Rwork | 0.178 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdy |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.793 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (0.6.3) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.920 | 48.460 | 2.390 |
| High resolution limit [Å] | 2.310 | 8.950 | 2.310 |
| Rmerge | 0.087 | 0.048 | 0.946 |
| Rmeas | 0.095 | 0.052 | 1.023 |
| Rpim | 0.036 | 0.020 | 0.386 |
| Total number of observations | 3917 | 22411 | |
| Number of reflections | 33259 | 644 | 3213 |
| <I/σ(I)> | 12.5 | 30 | 2.4 |
| Completeness [%] | 100.0 | 99.4 | 99.9 |
| Redundancy | 6.8 | 6.1 | 7 |
| CC(1/2) | 0.998 | 0.998 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 295 | 0.1 M HEPES pH 7.5, 20 %(w/v) PEG 4000, 10 %(w/v) Isopropanol |
