7KED
RNA polymerase II elongation complex with unnatural base dTPT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.890, 223.010, 194.700 |
Unit cell angles | 90.00, 101.60, 90.00 |
Refinement procedure
Resolution | 82.597 - 3.600 |
R-factor | 0.2668 |
Rwork | 0.266 |
R-free | 0.30540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e2j |
Data reduction software | MOSFLM |
Data scaling software | MOSFLM |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX (1.13) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 96.260 | 96.260 | 3.620 |
High resolution limit [Å] | 3.550 | 18.450 | 3.550 |
Rmerge | 0.317 | 0.079 | 1.503 |
Rmeas | 0.382 | 0.094 | 1.819 |
Rpim | 0.210 | 0.051 | 1.007 |
Total number of observations | 267013 | 2257 | 14436 |
Number of reflections | 82834 | 635 | 4583 |
<I/σ(I)> | 2.3 | 5.2 | 0.8 |
Completeness [%] | 96.9 | 98.6 | 97 |
Redundancy | 3.2 | 3.6 | 3.1 |
CC(1/2) | 0.893 | 0.967 | 0.322 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |