7KD6
Insulin Receptor L1-CR plus alphaCT fragment in co-complex with Fv 83-7 and single-chain insulin SCI-b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.670, 128.430, 148.790 |
Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
Resolution | 45.650 - 2.600 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oga |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.670 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.216 | 3.320 |
Number of reflections | 112001 | 11764 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 88.2 | |
Redundancy | 3.8 | |
CC(1/2) | 0.990 | 0.168 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 16% PEG 3350, 0.2 M sodium thiocyanate (NaSCN), 0.02% sodium azide (NaN3) |