7KD6

Insulin Receptor L1-CR plus alphaCT fragment in co-complex with Fv 83-7 and single-chain insulin SCI-b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	97.670, 128.430, 148.790
Unit cell angles	90.00, 90.18, 90.00

Refinement procedure

Resolution	45.650 - 2.600
R-factor	0.191
Rwork	0.189
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oga
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.670	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.216	3.320
Number of reflections	112001	11764
<I/σ(I)>	6.1
Completeness [%]	88.2
Redundancy	3.8
CC(1/2)	0.990	0.168

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	16% PEG 3350, 0.2 M sodium thiocyanate (NaSCN), 0.02% sodium azide (NaN3)