7KBT

Factor VIII in complex with the anti-C2 domain antibody, G99

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	132.243, 132.243, 380.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.480 - 4.150
R-factor	0.302
Rwork	0.300
R-free	0.33840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mf0
RMSD bond length	0.005
RMSD bond angle	1.074
Data reduction software	Adxv
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.480	4.298
High resolution limit [Å]	4.150	4.150
Rmerge	0.064	1.359
Rmeas	0.090	1.922
Rpim	0.064	1.359
Number of reflections	26284	2546
<I/σ(I)>	6.31	0.48
Completeness [%]	99.1	98.31
Redundancy	2	2
CC(1/2)	0.998	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8 - 18% PEG 1500, PEG 6K, or PEG 10K and 50mM malic acid, pH 7.