7KBA
Crystal structure of the HCMV pentamer-specific Fab 2-18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-10-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.080, 59.103, 141.927 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.020 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 26602 | 3773 |
<I/σ(I)> | 4 | 1.7 |
Completeness [%] | 98.8 | 96.7 |
Redundancy | 4.1 | |
CC(1/2) | 0.851 | 0.291 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | Seed crystals were grown in 0.1M HEPES pH 7.5, 45% PEG 400. Crystals were then seeded into 0.2M ammonium acetate, 0.1M sodium citrate tribasic dihydrate pH 5.6, 30% PEG 4000 |