7K8G
Crystal structure of bovine RPE65 in complex with 4-fluoro-MB-004 and palmitate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979358 |
Spacegroup name | P 65 |
Unit cell lengths | 175.511, 175.511, 86.657 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.880 - 1.950 |
R-factor | 0.19472 |
Rwork | 0.194 |
R-free | 0.21105 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4rsc |
RMSD bond length | 0.002 |
RMSD bond angle | 1.168 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.163 | 2.346 |
Number of reflections | 110605 | 17591 |
<I/σ(I)> | 11 | |
Completeness [%] | 99.7 | |
Redundancy | 11 | |
CC(1/2) | 0.998 | 0.463 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | Same as for entry 4RSC |