7K88
Crystal structure of bovine RPE65 in complex with hexaethylene glycol monooctyl ether
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-02 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979300 |
| Spacegroup name | P 65 |
| Unit cell lengths | 175.917, 175.917, 86.314 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.900 - 2.100 |
| R-factor | 0.19071 |
| Rwork | 0.189 |
| R-free | 0.22040 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4rse |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.204 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.116 | 1.703 |
| Number of reflections | 88260 | 14309 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5.2 | |
| CC(1/2) | 0.996 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | Same as for entry 4RSE |
