7K4H
Human Arginase 1 in complex with compound 04.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.140, 286.090, 66.952 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 48.880 - 1.650 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v7e |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.450 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.523 | 1.673 |
| High resolution limit [Å] | 1.645 | 1.645 |
| Number of reflections | 218547 | 11371 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 90.9 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.970 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% MMT (pH 7.0) 0.1 M ammonium formate, 16-22% PEG 8000 |
