7K17
Re-refined crystal structure of DNA-dependent protein kinase catalytic subunit complexed with Ku80 C-terminal helix
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 169.120, 132.640, 296.590 |
Unit cell angles | 90.00, 105.53, 90.00 |
Refinement procedure
Resolution | 49.920 - 4.300 |
R-factor | 0.287 |
Rwork | 0.286 |
R-free | 0.33530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7k0y |
RMSD bond length | 0.004 |
RMSD bond angle | 0.861 |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.918 | 4.407 |
High resolution limit [Å] | 4.300 | 4.300 |
Rmerge | 0.160 | 0.936 |
Number of reflections | 86508 | 0 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 97.8 | |
Redundancy | 35.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, 200 mM NaCl, 30% glycerol, 10 mM EDTA, 18% PEG 8000 | |
2 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, 200 mM NaCl, 30% glycerol, 10 mM EDTA, 8% PEG 8000 |